FIGURE 2.

In vitro screening pipeline to evaluate 2-aminoimidazole molecules as potential non-antibiotic therapeutics for C. difficile infection. Flowchart is designed as an in vitro screening pipeline to evaluate small molecules against C. difficile. The gray boxes represent the different protocols used to determine how the small molecules affect different stages of C. difficile life cycle (growth, toxin, and sporulation) and other members of the gut microbiota. Based on the results, at each box a decision is made whether to advance molecules for further screening with the goal of identifying suitable molecules to be advanced to mouse model testing.