FIGURE 3.

2-aminoimidazole molecules inhibit growth and toxin activity of C. difficile. (A) Inhibition of C. difficile R20291 growth (OD₆₀₀) in BHI media supplemented with small molecules (Compound 1 through 11) at a concentration of 10 μg/ml, solvent 0.25% DMSO (DMSO), or 2 μg/ml Vancomycin (Vanco). (B) Culture supernatants after 24 h growth inhibition assays were used for Vero cell cytotoxicity assays to measure toxin activity. Toxin titer is expressed as log10 reciprocal dilution toxin per 100 μl of C. difficile culture supernatant. Data presented represents mean ± SEM of triplicate experiments. In (B) statistical significance between positive control (solvent) and treatment groups was determined by Student’s parametric t-test with Welch’s correction (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001).