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. 2018 Jun 6;9:1206. doi: 10.3389/fmicb.2018.01206

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Copyright © 2018 Thanissery, Zeng, Doyle and Theriot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2-aminoimidazole molecules do not alter C. difficile sporulation. Sporulation of C. difficile on 70:30 agar plates after supplementation with 2-AI molecules (Compounds 1, 2, 3, 4, 7, 9, and 11) at a concentration of 10 μg/ml when compared to solvent 0.25% DMSO (DMSO, positive control), or 2 μg/ml vancomycin (Vanco, negative control) after 24 h. Data represents (A) Total vegetative cells and spores, and spores only, (B) Total vegetative cells and spores, (C) Spores only at 24 h post treatment.