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. 2018 Jun 12;38(3):BSR20180394. doi: 10.1042/BSR20180394

Figure 3. SRC-1 interacts with NF-kB for transcription of VEGFC.

Figure 3

(A) The predicted p65 binding sites in the promoter region of VEGFC. (B) A luciferase reporter vector with the full-length promoter construct of VEGFC containing mutations at p65 binding sites was transfected into TPC-1/shRNA-SRC-1 or shRNA-NC cells, and the luciferase activity was determined. (C) Biotin-labeled oligonucleotide probes corresponding to the VEGFC promoter formed complexes with p65 in the presence or absence of anti-p65 antibodies or specific/mutant competitors. These complexes were detected by EMSA. (D) The SRC-1/NF-kB (p50/p65) complexes in TPC-1 cells were precipitated by an anti-SRC-1 antibody, and the p50/p65 levels were determined. *P<0.05; data represent the mean ± S.D.