FIGURE 6.

Mitochondrial localization of WP3. (A) Confocal microscopy image of wild-type rice stably expressing a WP3-GFP protein in root tip. (B) Co-localization of WP3-GFP and mitotracker red in rice root cells. Mitochondria were indicated by arrows. Scale bars = 5 μm. (C) Immunoblotting detection of WP3 in rice mitochondria fraction. Mitochondria fractions and total proteins from rice stably expressing WP3-GFP were prepared, separated in SDS-PAGE, transferred to membranes, and immunoblotted using antibodies as indicated. WT whole, whole leaf lysates from wild type plant; WT mito., mitochondrial fraction from wild-type plant; WP3-GFP whole, whole leaf lysates from WP3-GFP transgenic plant; WP3-GFP mito., mitochondrial fraction from WP3-GFP transgenic; WP3-GFP without mito., remained fractions after mitochondrial isolation from WP3-GFP transgenic plants.