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. 2018 Jun 6;9:762. doi: 10.3389/fpls.2018.00762

FIGURE 8.

FIGURE 8

Semi-quantitative RT-PCR amplification of indicated genes related to mitochondria and chloroplasts development in wild type and wp3 mutant. (A) 0.3–1 cm inflorescences were collected to prepare cDNA for amplification of mitochondria related gene. Actin was used for an internal control. AOX1a, alternative oxidase 1a; CytC1-1, cytochrome c1-1; COX1, cytochrome c oxidase subunit 1; SDH, succinate dehydrogenase; TOM40, translocase of outer mitochondrial membrane 40; UCP1, uncoupling protein 1; VDAC, voltage-dependent anion-selective channel. (B) RT-PCR analyses of chloroplast associated gene expression. Three-week-old plant leaves were collected for cDNA preparation. The white and green sectors in wp3 stripe leaves were harvested separately. RBCL, the large subunit of chloroplast ribulose bisphosphate carboxylase; RBCS, the small subunit of chloroplast ribulose bisphosphate carboxylase; RPL23, 50S ribosome protein L23; CAB, the light-harvesting chlorophyll alb protein; OsSigA, plastid RNA polymerase sigma factor; OsFtsZ, plastid division protein FtsZ. The experiments were performed in three biological replicates and the representative results were shown.