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. 2018 Jun 6;9:540. doi: 10.3389/fphar.2018.00540

FIGURE 5.

FIGURE 5

Puerarin protected against AngII induced-cardiac fibrosis and oxidative stress in cultured neonatal rat cardiac fibroblasts. (A–D) Representative western blots and quantitative analysis of Nrf2, UGT1A1, collagen I, and collagen III. (A) The time courses of puerarin effects on cultured neonatal rat cardiac fibroblasts. (B) The time courses of effects of tBHQ (an agonist of Nrf2) on cardiac fibrosis. (C) Puerarin protected against AngII induced-cardiac fibrosis. (D) The time courses of effects of tBHQ and Brusatol (an inhibitor of Nrf2) on AngII induced-cardiac fibrosis. (E) Puerarin protected against AngII induced- intracellular ROS (by fluorescence microscopy and fluorometer). (F) Puerarin inhibited the expression of Keap1 in cultured neonatal rat cardiac fibroblasts. Representative western blots and quantitative analysis of Keap 1. AngII, 1 μM; puerarin, 100 μM; tBHQ, 50 nM; Brusatol, 100 nM; siNrf2, Nrf2 specific siRNA. ∗P < 0.05 vs. control (0 h), ∗∗P < 0.01 vs. control (0 h), #P < 0.05 vs. AngII, ##P < 0.01 vs. AngII, ▵ P < 0.05 vs. Pue + AngII, ▵ ▵ P < 0.01 vs. Pue + AngII. n = 6 for each group.