Fig. 3.

LCMS chromatogram of Caesalpinia sappan L. heartwood methanol extract. 10 µL of sample was loaded in eclipse XDB C18 column (150*4.6 mm and 5µ pore size), with 1.0 mL/min flow rate of Methanol:Water (80:20) as mobile phase. Mass spectra was performed by ESI (Electro spray Ionization), the formation of positive and negative ions occurs in high yield which is useful for determination of compounds.