Fig. 3.

Primary atrial Fib from subjects without AF were left untreated (CTR), exposed to thrombin (Th) and/or dabigatran (Dab), or transfected with a control (siCTR) or a PAR1-targeting (siPAR1) siRNA with or without subsequent incubation with Th or Dab. At the end of treatments, cells were stained with Picro-Sirius red. Representative images of the staining in conventional and polarized light microscopy are given in (A) and (B), respectively, while quantification of the red signal in conventional light microscopy is presented in (C). Magnification is 200× and bars correspond to 50 μm. Data in the graph are from three independent replicates and were compared by using the Kruskal-Wallis test.