Table 1.
Primer sequences for 16 miRNA evaluated. Forward and RT stem-loop primers and an universal reverse primer.
| miRNA ID | miRNA sequence | Primer type | Primer sequences |
|---|---|---|---|
| miR132-3p | uaacagucuacagccauggucg | Forward | TCC GGC TAA CAG TCT ACA GCC A |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC cgacca | ||
| miR138-5p | agcugguguugugaaucaggccg | Forward | TCC GGA AGC TGG TGT TGT GAA TC |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC cggcct | ||
| miR125a-5p | ucccugagacccuuuaaccuguga | Forward | GTC GCG ATC CCT GAG ACC CTT TA |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC tcacag | ||
| miR195-5p | uagcagcacagaaauauuggc | Forward | GGG CGC TAG CAG CAC AGA AAT A |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC gccaat | ||
| miR199a-5p | cccaguguucagacuaccuguuc | Forward | GAT GCG CCC AGT GTT CAG ACT |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC gaacag | ||
| miR134-5p | ugugacugguugaccagagggg | Forward | GGC TCT TGT GAC TGG TTG ACC A |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC cccctc | ||
| miR124-3p | uaaggcacgcggugaaugcc | Forward | CTA GCT TAA GGC ACG CGG TGA |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC ggcatt | ||
| miR181a-5p | aacauucaacgcugucggugagu | Forward | GCG CTG AAC ATT CAA CGC TGT C |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC actcac | ||
| miR181b-5p | aacauucauugcugucggugggu | Forward | GCT GCG CAA CAT TCA TTG CTG TC |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC acccac | ||
| miR25-3p | cauugcacuugucucggucuga | Forward | TCA GCA CAT TGC ACT TGT CTC GG |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC tcagac | ||
| miR21-5p | uagcuuaucagacugauguuga | Forward | CCG GCG CTA GCT TAT CAG ACT GAT |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC tcaaca | ||
| miR23a-3p | aucacauugccagggauuucc | Forward | GCT GTC ATC ACA TTG CCA GGG A |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC ggaaat | ||
| miR146a-5p | ugagaacugaauuccauggguu | Forward | CGT GGC GTG AGA ACT GAA TTC CA |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC aaccca | ||
| miR218-5p | uugugcuugaucuaaccaugu | Forward | GCC GTC CTT GTG CTT GAT CTA ACC |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC acatgg | ||
| miR30c-5p | uguaaacauccuacacucucagc | Forward | GCG TCG CTG TAA ACA TCC TAC ACT C |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC gctgag | ||
| miR191-5p | caacggaaucccaaaagcagcug | Forward | GGA GCG TCA ACG GAA TCC CAA AAG |
| RT stem-loop | GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG AC cagctg | ||
| Reverse Universal Primer | Reverse | CCA GTG CAG GGT CCG AGG TA |