Subject area	Pharmacology, Toxicology and Pharmaceutical Science
More specific subject area	Autism Spectrum Disorder
	Toxicology
	Natural products
Type of data	Table, Figures
How data was acquired	Animal behavior analysis and Reverse Transcription followed by Quantitative Polymerase Chain Reaction (RT-qPCR)
Data format	Analyzed
Experimental factors	The animal model of autism was induced by a single intraperitoneal injection of 600 mg/kg VPA or saline solution on embryonic day 12.5 (E12.5). RSV treatments were achieved by daily subcutaneous injections of RSV (3.6 mg/kg) or DMSO from E6.5 to E18.5. Blood samples from these animals were obtained by cardiac puncture 30 days after birth. Peripheral blood samples from autistic male individuals and from the control group (5–15 years-old range) were obtained at Clinical Hospital of Porto Alegre (HCPA).
Experimental features	Social Transmission of Food Preference (STFP) test was performed in male rats after food habituation, consumption of one of flavored food by demonstrator rat and interaction between demonstrator and observer rats. The amount of cued and non-cued food eaten by observers from each litter was weighed and recorded. After homogenization of blood samples, we performed RNA extraction and the mature miRNA expression was evaluated by reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR), using fluorescence of SYBR Green to detect amplification, estimate Ct values and to determine specificity after melting curve analysis.
Data source location	Department of Biochemistry, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil. Clinical Hospital of Porto Alegre, Porto Alegre, RS, Brazil.
Data accessibility	Hirsch, M. M. et al. Behavioral alterations in autism model induced by valproic acid and translational analysis of circulating microRNA. Food Chem. Toxicology 115 (2018): 336–343. doi: 10.1016/j.fct.2018.02.061.[1]