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Oligonucleotide primers and probes for real-time PCR.
| Primers and probes | Sequence (5′–3′) | Production (bp) | Target |
|---|---|---|---|
| A. actinomycetemcomins | |||
| Forward | TTGATCGTGCGAGAATGCTT | ||
| Reverse | ATCGCCGTTATAACCAAATTTCTT | ||
| Probe | FAM-AGGAATACTCGAAACGC-MGB | 65 | lktA |
| P. gingivalis | |||
| Forward | TACCCATCGTCGCCTTGGT | ||
| Reverse | CGGACTAAAACCGCATACACTTG | ||
| Probe | FAM-ATTTATAGCTGTAAGATAGGC-MGB | 126 | 16S rRNA |
