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. 2018 Jun 13;9:2306. doi: 10.1038/s41467-018-04702-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Single-molecule experiments on helicase and non-replicating DNAP collision with a TEC. a Distributions of DNA length increase rates of helicase unwinding with or without replication in 0.5 mM dNTP (each) under 5 pN of force. b Experimental design. Left panel shows a cartoon of E. coli TEC that was stalled at +20 nt from the promoter while a helicase with a non-replicating DNAP encountered the TEC co-directionally. c A representative trace of unwinding without replication after collision. Experiments were carried out in the presence of helicase, DNAP, and 0.5 mM dNTP (each) under 5 pN of force. The dotted line indicates the expected stalled TEC position. The cartoon on the top illustrates replication status after the collision with RNAP. d A representative trace of unwinding with replication after collision. Same experimental conditions were used as in c. For clarity, traces have been shifted along the time axis. e Distributions of DNA length increase rates before (upper panel) and after (lower panel) the collision with RNAP