Fig. 5.

Angptl4^−/− mice have reduced liver fat. a Body weight, (b) lean and (c) fat mass in Angptl4^−/− and littermate control mice on a high-fat diet. d Liver and (e) epididymal white fat weights were evaluated in mice described in (a–c) at the time of killing. f Hepatic triglyceride levels and (g) neutral lipid staining in the livers described in (d), scale bar = 200 µm. h Respiratory exchange ratio (RER), (i) locomotor activity, (j) food intake, and (k) energy expenditure were measured during dark and light cycles in Angptl4^−/− and littermate control mice on a high-fat diet. All values are mean ± SEM. Statistical analysis was conducted by Welch’s t-test; ^*p < 0.05; ^**p < 0.01. The study was conducted in two different cohorts of mice, with qualitatively similar results in each replicate