Table 1.

Selected candidate reference genes, their corresponding product name, primer sequences (annealing temperature of 60 °C), amplicon size in base pairs (bp), their respective PCR amplification efficiencies and the mean C_T values(±standard deviation) assessed in K. pneumoniae cells submitted to various experimental conditions and at different phases of growth.

Genes	Product Name	Forward and Reverse primer sequences (5′ > 3′)	Amplicon size	R2	E (%)	Mean CT ± SD
proC	Pyrroline-5-carboxylate reductase	GATTGCCGATATCGTCTTCG GAGACCACCAGCGACTCTTT	99 bp	0.989	83.4	24.91 ± 0.62
glnA	Glutamine synthetase	GAAGGCGGTAACAAAGGTCA TACACATGGTGGAACGGATG	97 bp	0.988	98.2	23.79 ± 1.19
gyrA	DNA gyrase subunit A	GTGACCCGTCGTACGATTTT GATAATCGGGTCGATGTTGG	99 bp	0.987	96.6	24.45 ± 1.10
recA	Recombinase A	TTAAACAGGCCGAATTCCAG CCGCTTTCTCAATCAGCTTC	99 bp	0.989	92.1	22.40 ± 0.61
rpoD	RNA polymerase sigma factor RpoD	TCCGGTGCATATGATTGAGA ATACGCTCAGCCAGCTCTTC	105 bp	0.989	87.5	25.05 ± 0.80
rho	Transcription termination factor Rho	AACTACGACAAGCCGGAAAA ACCGTTACCACGCTCCATAC	99 bp	0.998	92.4	23.92 ± 0.90
rpoC	DNA-directed RNA polymerase subunit beta’	TATTCTGGTTCCACGCAACA GGATACAACGGAACGCACTT	97 bp	0.969	91.2	23.28 ± 0.88
rrsH	16S ribosomal RNA	GACGATCCCTAGCTGGTCTG GTGCAATATTCCCCACTGCT	95 bp	0.957	94.2	10.60 ± 0.55
aat	Leucyl/phenylalanyl-tRNA-protein transferase	CTGGATAACCAGCAGTATCGTTC GTACATTCCACCTACCAGCGTATT	106 bp	0.994	84.1	38.83 ± 1.55
ffh	Signal recognition particle protein	GCTAAGCCGGAAATCATCAA ATGTCGTCGAACTGCTTGAG	104 bp	0.986	97.2	23.93 ± 1.39
trpS	Tryptophanyl-tRNA synthetase II	GCCACTGTAAGGCGCTACTC GCCGATAACGTCAGCGTATT	100 bp	0.992	87.1	29.14 ± 1.50

R²: Correlation coefficient; E: PCR efficiency (%); S.D.: standard deviation.