Figure 3.

Comparison of TMRM intensity between control and patient lines. TMRM fluorescence intensity measured in the whole cell (A) or in mitochondria (E) in two control lines and two patient lines, normalized to Control 1 p < 0.05–p < 0.0001 Kruskal-Wallis test and relative frequency distribution of same data (B and F). Intensity data from both control lines and both patient lines combined measured in the whole cell (C) or in mitochondria (G), p < 0.0001 Kolmogrov-Smirnov test. Change in intensity in CCCP treated cells, normalized to basal for each line at a cellular level (D) and a mitochondrial level (H), p < 0.0001 compared to basal for each line, Kruskal-Wallis test. Dots represent mean data for each image, line and bars represent median ± interquartile range, n = 3 independent experiments.