Table 3.
Predicted binding sides cloned into a pmirGlo Dual Luciferase miRNA target expression vector.
| Target mRNA_nr. of binding site | Location in 3′UTR (nt) | Binding sequence | |
|---|---|---|---|
| RAB8A | 95–101 | 3′ acgacaaucgGGaU-CGGGGCGu 5′ 5′ ccucccaccuCCaAcGCCCCGCc 3′ |
hsa-miR-744 RAB8A |
| IP6K | 2773–2781 | 3′ acgacaAUCGgGAu---CGGGGCGu 5′ 5′ uacaccUAGCgCUgcccGCCCCGCu 3′ |
hsa-miR-744 IP6K |
| ILK_1 | 77–98 | 3′ acGACaAuCGGgaucGGGGCGu 5′ 5′ cuCUGgUuGCCu--cCCCCGCc 3′ |
hsa-miR-744 ILK |
| ILK_2 | 226–232 | 3′ acgACaaUCgggA-UCGGGGCGu 5′ 5′ acaUGggAGggaUcAGCCCCGCc 3′ |
hsa-miR-744 ILK |
| HNRNPC | 268–276 | 3′ acgacAAUcggG-AU--CGGGGCGu 5′ 5′ guucaUUAauuCaUAuuGCCCCGCg 3′ |
hsa-miR-744 HNRNPC |
| PRKCD | 95–102 | 3′ acGAcaAucggGAuCGGGGCGu 5′ 5′ ugaCUucUgcugCUgGCCCCGCc 3′ |
hsa-miR-744 PRKCD |
| ADAR1 | 866–888 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ uccuaggaauauuuuCCCCGCc 3′ |
hsa-miR-744 ADAR |
| NFIX_1 | 185–206 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ cuccagcccggggacCCCCGCg 3′ |
hsa-miR-744 NFIX |
| NFIX_2 | 347–368 | 3′ acgacaaucGGGAucGGGGCGu 5′ 5′ ccuuccuccCCCUgaCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_3 | 920–941 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ cgggccccaggucgcCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_4 | 1198–1218 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ ugccccccgacuuucCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_5 | 1206–1227 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ gacuuucccccgccuCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_6 | 2511–2517 | 3′ acgacaauCgGGAuCGGGGCGu 5′ 5′ aagaaccgGgCCU-GCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_7 | 3058–3064 | 3′ acgacaauCGGGaucGGGGCGu 5′ 5′ gguccccgGCCCcucCCCCGCa 3′ |
hsa-miR-744 NFIX |
| NFIX_8 | 3358–3365 | 3′ acgacaaucgggauCGGGGCGu 5′ 5′ cacgcacgcacacgGCCCCGCa 3′ |
hsa-miR-744 NFIX |
| NFIX_9 | 3370–3376 | 3′ acgacaaucgggaUCGGGGCGu 5′ 5′ cggccccgcacacAGCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_10 | 3381–3387 | 3′ acgacaaucgggaucGGGGCGu 5′ 5′ cacagccccgccccaCCCCGCa 3′ |
hsa-miR-744 NFIX |
| NFIX_11 | 3404–3425 | 3′ acGAcaAUcgGGaucGGGGCGu 5′ 5′ gcCUuaUAcaCCcgcCCCCGCg 3′ |
hsa-miR-744 NFIX |
| NFIX_12 | 3636–3642 | 3′ acgacaaucGGGaUCGGGGCGu 5′ 5′ cgaggggcaCCCcAGCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_13 | 3687–3693 | 3′ acgacaaucGGGAucGGGGCGu 5′ 5′ ggggcgcccCCCUccCCCCGCa 3′ |
hsa-miR-744 NFIX |
| NFIX_14 | 3709–3715 | 3′ acgacaAuCgGGaUCGGGGCGu 5′ 5′ ccagccUgGgCC-AGCCCCGCu 3′ |
hsa-miR-744 NFIX |
| NFIX_15 | 4058–4064 | 3′ acGACAAucGgGA-uCGGGGCGu 5′ 5′ guCUGUUccCuCUccGCCCCGCc 3′ |
hsa-miR-744 NFIX |
| NFIX_16 | 4068–4074 | 3′ acgacaauCGGGauCGGGGCGu 5′ 5′ uccgccccGCCC-cGCCCCGCc 3′ |
hsa-miR-744 NFIX |