Table 2.
Diffusion times (tD), derived diffusion coefficients (Dc), hydrodynamic diameters (dH) and relative cross-correlation FCCS/ACF_G. The diffusion times refer to the curves in Fig. 3. NPs were labeled with ATTO633. Measurements were performed with NPs with PC after the excess of BSA was removed from the dispersion by centrifugation. ConA was added to the PC and measurements were performed in situ.
| tD [µs]a | Dc [µm2/s]b | dHc [nm] | FCCR/ACF_G | tD [µs]a | Dc [µm2/s]b | dHc [nm] | FCCR/ACF_G | ||
|---|---|---|---|---|---|---|---|---|---|
| PC BSA | 1870 ± 80 | 5.2 ± 0.2 | 65.6 ± 2.5 | — | PC BSAd | 1163 ± 230 | 8.4 ± 1.5 | 40.6 ± 7.5 | 0.17 |
| +ConAd 5 min | 1242 ± 56 | 7.9 ± 0.3 | 43.2 ± 1.6 | 0.73 | +ConA 5 min | 2936 ± 180 | 3.3 ± 0.1 | 103.4 ± 3.1 | 0.12 |
| +ConAd 90 min | 762 ± 67 | 12.9 ± 1.0 | 26.4 ± 2.1 | 0.63 | +ConA 90 min | 1162 ± 47 | 8.4 ± 0.2 | 40.6 ± 1.0 | 0.03 |
| +ConAd 120 min | 1186 ± 42 | 8.2 ± 0.4 | 41.6 ± 2.1 | 0.53 | +ConA 120 min | 1287 ± 38 | 7.6 ± 0.2 | 45.0 ± 1.2 | 0.05 |
(a) Values obtained fitting the raw data by Global fitting of 1-componet 3D-Normal Diffusion function. (b) The diffusion coefficient was calculated from equation Equation S4 in SI. (c) Obtained applying the Stoke-Einstein equation to Dc. (d) Labeled by ATTO488.