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. 2018 Jun 13;8:9028. doi: 10.1038/s41598-018-27334-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Schematic representation of the 100 bp deletion in the S. anginosus ∆ccpA strain. The site of deletion is marked with black lines and the deleted base pairs in bold. The 100 bp deletion results in a frameshift and a premature stop codon (*). (b) Effect of glucose supplementation on SLS promoter activity. The activity of the SLS promoter was determined using an EGFP reporter plasmid. The relative mean fluorescence intensity (MFI) of cells grown in THY medium supplemented with the indicated glucose concentrations is shown in comparison to the positive control. Negative control: S. anginosus pBSU409; positive control: S. anginosus pBSU409::cfbprom; WT: S. anginosus type strain; ΔccpA: S. anginosus ΔccpA strain. The mean values and standard deviations of five independent experiments are shown. Mann-Whitney-U test was performed to illustrate significant difference to S. anginosus pBSU409::sagprom (p < 0.05).