Table 1.
Strains and plasmids.
| Strain or plasmid | Definition | Source |
|---|---|---|
| S. anginosus | ||
| BSU 458 | S. anginosus type strain ATCC33397, Hly+ | ATCC |
| BSU 554 | BSU 458 derivative, carrying pBSU409 | 44 |
| BSU 556 | BSU 458 derivative, carrying pBSU409::cfbprom | 45 |
| BSU 805 | BSU 458 derivative, carrying pBSU409::sagprom | 11 |
| BSU 926 | BSU 458 derivative; ∆ccpA | This study |
| BSU 928 | BSU 458 derivative; ∆ccpA, carrying pBSU409::sagprom | This study |
| BSU 948 | BSU 928 derivative; ∆ccpA::ccpA | This study |
| BSU 886 | BSU 458 derivative; carrying pBSU409::sagprom_creA_del | This study |
| BSU 913 | BSU 458 derivative; carrying pBSU409::sagprom_creB_del | This study |
| BSU 916 | BSU 458 derivative; carrying pBSU409::sagprom_creC_mut | This study |
| E. coli | ||
| EC101 | E. coli JM101 derivative with repA from pWV01 integrated into the chromosome | 46 |
| DH5α | endA1 hsdR17 supE44 DlacU169(f80lacZDM15) recA1 gyrA96 thi-1 relA1 | Boehringer |
| BL21(DE3) | E. coli BL21 derivative with DE3 λ prophage carrying the T7 RNA polymerase gene and lacIq | Novagen |
| BSU 957 | BL21 derivative, carrying pET21a-ccpA-N-His | This study |
| pAT18 | lacZα, ori pUC, ori pAmβ1, EmR | 47 |
| pAT28 | lacZα, ori pUC, ori pAmβ1, SpcR | 48 |
| pBSU409 | pAT28 derivative carrying egfp | 44 |
| pBSU803 | pBSU409::sagprom; pBSU409 derivative, egfp under the control of the SLS promoter | 11 |
| pBSU881 | pBSU409::sagprom_creA_del; pBSU803 derivative carrying a deletion of creA | This study |
| pBSU880 | pBSU409::sagprom_creB_del; pBSU803 derivative carrying a deletion of creB | This study |
| pBSU876 | pBSU409::sagprom_creC_mut; pBSU803 derivative carrying mutations in creC | This study |
| pET21a | lacI, ori F1, ori pBR322, T7 promoter and terminator, AmpR | Novagen |
| pET21a-ccpA-N-His | pET21a derivative carrying ccpA | This study |
| pG+host5-ccpA∆100nt | pG+host5 derivative carrying up- and downstream sequences of ccpA | This study |