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. 2018 Jun 13;8:9042. doi: 10.1038/s41598-018-27086-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GO term enrichment in the clusters from C10. In cluster 1, the genes that had been downregulated by OS appeared to have normal expression levels in the presence of LB. In cluster 2, the genes that had been upregulated by OS appeared to have normal expression levels in the presence of LB. In cluster 3, the genes were upregulated by OS and downregulated in the presence of LB. In cluster 4, the genes were downregulated by OS and upregulated in the presence of LB. PANTHER tools (http://pantherdb.org/) were used to test the GO term enrichment in gene sets, relative to the background list of all the genes in the amoeba genome. The GO terms were used to identify enriched molecular processes; those with an FE ≥ 2.5 were plotted. A summary for gene ontology (GO) term analysis is provided in Tables 2 and S5.