Figure 3.

hPSCs Give Rise to Multiple DS-RGC Subtypes

(A and B) ON-OFF DS-RGCs were identified by the co-expression of the RGC marker BRN3 with either (A) CART or (B) CDH6.

(C) ON DS-RGCs were identified by the expression of FSTL4 co-localized with BRN3.

(D) Quantification of immunocytochemistry results identified the expression of CART, CDH6, and FSTL4 within the BRN3-RGC population at 25.95% ± 0.46%, 17.16% ± 0.51%, and 30.49% ± 0.58%, respectively.

(E) Single-cell qRT-PCR analysis demonstrated the combinatorial expression of DS-RGC markers, in conjunction with RGC-associated markers. Error bars represent the SEM (n = 36 technical replicates from 3 biological replicates for each bar using miPS2, H9, and H7 cell lines).

Scale bars, 20 μm.