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. 2018 Mar 22;10(4):1282–1293. doi: 10.1016/j.stemcr.2018.02.010

Figure 7.

Figure 7

Identification and Confirmation of DCX as a DS-RGC Marker

(A–C) DCX was highly co-localized with (A) FSTL4, while its co-expression with pan-RGC markers (B) BRN3 and (C) SNCG demonstrated less correlation.

(D) Quantification of immunocytochemistry results indicated that DCX expression correlated with 82.48% ± 1.66% of FSTL4-positive RGCs, while it was identified in subsets of BRN3- and SNCG-positive RGCs at 42.61% ± 1.88% and 53.57% ± 1.88%, respectively.

(E) Single-cell RNA-seq values demonstrate expression of DCX correlated with other DS-RGC markers, but was found exclusive of markers of other RGC subtypes and retinal cells.

Scale bars, 50 μm. Error bars represent SEM (n = 30 technical replicates from 3 biological replicates for each bar using miPS2, H9, and H7 cell lines).