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. 2018 Mar 15;10(4):1412–1425. doi: 10.1016/j.stemcr.2018.02.009

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

CD73 Regulates the Expression of Stemness- and EMT-Related Genes and OC Cell Morphology

(A) CD73 knockdown OC primary cells (OC5) were analyzed for the expression of genes related to stemness and EMT. Data are expressed as the relative mRNA expression (2^−ΔΔCT) of reported genes in silenced cells as compared with control cells (dashed line). The experiment was performed in triplicate and data are expressed as means ± SD.

(B) Representative images of morphological changes in primary OC adherent cells after CD73 silencing. CD73 knockdown induced a phenotypic reversion from a mesenchymal to epithelial morphology in OC cells. Scale bars, 400 μm. See also Figure S5C.