FIGURE 1.

GEN prevents repression of BRCA-1 in breast cancer Michigan Cancer Foundation-7 cells with activated aromatic hydrocarbon receptor. Cells were cultured for 72 h in control medium containing E2 (10 nM) or TCDD (10 nM) (A) or E2 + TCDD and various concentrations of GEN (B) as described in Methods. Bands show immunocomplexes for BRCA-1 and the internal standard GAPDH. (C, D) Bars represent means ± SEMs of BRCA-1 expression (fold of control) from 4 (C) and 2 (D) separate experiments performed in duplicate. Labeled means without a common letter differ, P < 0.05. BRCA-1, breast cancer 1, early onset; E2, 17β-estradiol; GEN, genistein; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.