FIGURE 6.

Long-term reversal effects of GEN and EGCG on BRCA-1 expression in breast cancer Michigan Cancer Foundation-7 cells with activated aromatic hydrocarbon receptor. Cells were precultured for 48 h in control medium plus E2 (10 nM) or E2 + TCDD (10 nM). Then, media were washed out and cells were cultured for an additional 7, 8, and 9 d in the presence of E2 alone or E2 + 1 μM GEN (A) or EGCG (B). Bands show immunocomplexes for BRCA-1 and the internal standard GAPDH. (C) Bars represent means ± SEMs of BRCA-1 expression (fold of E2) from 3 separate experiments with 3 replicates. Labeled means without a common letter differ, P < 0.05. BRCA-1, breast cancer 1, early onset; EGCG, (–)-epigallocatechin-3-gallate; E2, 17β-estradiol; GEN, genistein; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.