FIGURE 9.

GEN and αNF inhibit cell proliferation in UACC-3199 cells with constitutively active aromatic hydrocarbon receptor. (A) UACC-3199 cells were cultured for 72 h in control medium and in the presence of E2 (10 nM), GEN (1 and 10 μM), and E2 + GEN or αNF (2 μM) and E2 + αNF. Bars represent means ± SEMs of quantitation (fold of control) of proliferation determined by MTT assay from 2 separate experiments with 5 replicates. (B) Bands show representative immunocomplexes for cyclin D1 and the internal standard GAPDH in UACC-3199 cells cultured for 72 h in control medium and after treatment with E2, αNF, E2 + αNF, or E2 + GEN. (C) Bars represent means ± SEMs of cyclin D1 quantitation (fold of control) from 2 independent experiments performed in duplicate. Labeled means without a common letter differ, P < 0.05. E2, 17β-estradiol; GEN, genistein; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; UACC, University of Arizona Cell Culture; αNF, α-naphthoflavone.