Fig. 2.

Purification of gt1a E1E2-Flag from transfected 293T cells. 48 hrs post transfection with gt1a E1E2-Flag expression plasmid, the cells were lysed and incubated with the M2 anti-Flag agarose. The eluted fraction was analyzed by A. Coomassie-stained SDS-PAGE (the E2 and E1 bands are indicated by red frames; a ~ 50 kDa band of the H chain of the anti-Flag antibody is visible as the only impurity) and by B. Western blot using the anti-E2 AP33 and the anti-E1 A4 mAbs.