Fig. 5.

Neutralization of HCVcc by the E1E2-Flag polyclonal mouse sera. Huh-7-J20 cells were infected with 50 FFU of virus, pre-incubated with the appropriate inhibitory or control antibody (H77/JFH1/HQL chimera – left panel; JFH1-AM71 – right panel). After 48 hrs, the infectivity was determined by measurement of the SEAP activity released into the medium. Each dot on the plot represents one animal (■ - 1a-Flag, ♦ - 1b-Flag, • - 2a-Flag). The anti-E2 AP33 mAb and preIM serum were used as positive and negative controls, respectively. The horizontal bars represent the median value for each group.