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. 2018 Jun 12;17:94. doi: 10.1186/s12943-018-0843-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — excessive PTEN abrogates the oncogenic action of HULC. a Cells growthassay using CCK8. b Cell BrdU staining assay. c Cells colony formation assay. d Tumorigenesis test in vivo. The wet weight of tumor was determined for each mouse. e The appearance time of tumor was determined for each mouse. f PCNA staining (DAB staining, original magnification× 100). g positive rate of PCNA staining. h Cells growth assay using CCK8 in pCMV6-A-GFP control group, pCMV6-A-GFP-HULC group, and pCMV6-A-GFP-HULC+ 3-Methyladenine (3-MA). i Cells colony formation assay in pCMV6-A-GFP control group, pCMV6-A-GFP-HULC group, and pCMV6-A-GFP–HULC + 3-Methyladenine (3-MA)