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. 2018 Apr 10;10(4):1384–1397. doi: 10.1016/j.stemcr.2018.03.006

Figure 3.

Figure 3

PLAG1 and USF2 Are Required for MSI2 Promoter Activity and Expression

(A and B). Knockdown efficiencies of shUSF2 (E) and shPLAG1 (F) as measured post-infection by q-RT-PCR and western blot where sufficient cells were available (inset, A) in K562 cells (n = 3 experiments for each hairpin).

(C) MSI2 transcript level reductions following USF2 and PLAG1 knockdown (n = 3 experiments).

(D) Western blot (inset) validation of overexpression of the dominant-negative forms of PLAG1-B (PLAG1B-DN) and PLAG1-S (PLAG1S-DN), and graph of MSI2 transcript levels following introduction of PLAG1B-DN or PLAG1S-DN into K562 cells. (n = 3 experiments).

Data are presented as means ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Table S3.