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. 2018 May;13(5):827–836. doi: 10.4103/1673-5374.232477

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Copyright: © 2018 Neural Regeneration Research

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Elevated endoplasmic reticulum stress response pathway-related proteins in the injured cortex post-TBI (western blot assay).

The injured-side cortex of rat brain was isolated at the indicated times (1, 3, 6, 12, and 24 hours) post-TBI. (A) Expression of GADD153 and caspase-12 was detected by western blot assay. (B, C) Quantified grayscale intensity of GADD153 and caspase-12 expression detected by western blot assay. (D) Caspase-12 enzymatic activity was examined in cell lysates using the fluorogenic substrate, Ac-ATAD-AFC. Data are expressed as the mean ± SD (n = 6/group), and were analyzed by one-way analysis of variance combined with Tukey’s multiple-comparisons test. *P < 0.05, vs. sham group. TBI: Traumatic brain injury.