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. 2018 May;13(5):896–907. doi: 10.4103/1673-5374.232488

Figure 5.

Figure 5

Phenotyping of microglia/macrophages in ngr1+/+ and ngr1–/– EAE-induced mice at clinical scores 0 and 3.

(A) There was a significant increase in the percentage of the M1 macrophage/microglial cell phenotype in the spinal cord of EAE-induced ngr1+/+ mice at the peak-chronic stage of disease when compared with the M2 phenotype. In addition, there was a substantial increase in these M1-pathogenic populations compared to cells within the pre-onset stage. Results are expressed as the mean ± SEM, Student’s t-test, n = 3/clinical score, *P < 0.05, **P < 0.01, and ****P < 0.0001. (B) In the spinal cord of EAE-induced ngr1–/– mice, there was a significant elevation in M2 macrophage/microglial cell percentages, more so than in the M1 phenotype at clinical score 3, even though more M2 than M1 cells were present at clinical score 0. Data are expressed as the mean ± SEM, Student’s t-test, n = 3/clinical score, **P < 0.01 and ****P < 0.0001. EAE: Experimental autoimmune encephalomyelitis; M1: classically activated macrophages; M2: alternatively activated macrophages.