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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Stem Cells. 2018 Mar;36(3):349–362. doi: 10.1002/stem.2746

Figure 3. Intermediate differentiation in H9 cells is through the neural crest lineage.

Figure 3

(A, B) Double staining with antibodies against SOX10/PAX7, SOX10/TWIST1, and single stains with p75NTR, and PDGFRα at indicated time points of differentiation. Scale bar = 100 μm.

(C–E) Quantitative mRNA analysis of early lineage genes, normalized to GAPDH, n=3 independent samples ± SD.

T/BRA, T-Brachyury; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.