TABLE 1.
Screening of the polyphenolic fractions on osteoclast differentiation by using Raw 264.7 cells under normal and inflammatory conditions1
| Normal conditions (0 ng LPS/mL) | Inflammatory conditions (1 ng LPS/mL) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fraction | CON (0 μg/mL) | 0.1 μg/mL | 1 μg/mL | 10 μg/mL | P | CON (0 μg/mL) | 0 μg/mL | 0.1 μg/mL | 1 μg/mL | 10 μg/mL | P |
| DP-FrA | 100 ± 7a | 97 ± 11a | 82 ± 5b | 72 ± 6b | 0.049 | 100 ± 4b | 135 ± 7a | 106 ± 9b | 97 ± 3b | 93 ± 3b | 0.001 |
| DP-FrB | 100 ± 7 | 92 ± 7 | 82 ± 5 | 76 ± 6 | 0.099 | — | — | — | — | — | — |
| DP-FrC | 100 ± 7 | 80 ± 3 | 90 ± 6 | 82 ± 8 | 0.161 | — | — | — | — | — | — |
| DP-FrD | 100 ± 7 | 89 ± 6 | 86 ± 5 | 86 ± 5 | 0.051 | — | — | — | — | — | — |
| DP-FrE | 100 ± 7a | 79 ± 4b | 78 ± 6b | 74 ± 6b | 0.034 | 100 ± 4b | 135 ± 7a | 127 ± 2a | 103 ± 5b | 99 ± 3b | <0.001 |
| DP-FrF | 100 ± 7a | 79 ± 2b | 74 ± 2b,c | 66 ± 2c | 0.001 | 100 ± 4b | 135 ± 7a | 102 ± 5b | 100 ± 6b | 88 ± 4b | 0.001 |
Values are mean percentages of control ± SEs. Dried plum fractions (DP-FrA–DP-FrF) were screened for their ability to alter osteoclast differentiation under normal (0 ng LPS/mL) conditions. Fractions with significant effects under normal conditions (DP-FrA, DP-FrE, and DP-FrF) were tested under inflammatory conditions (1 ng LPS/mL). Data presented show osteoclast numbers from 2 to 3 experiments (n = 6 replicates/experiment) expressed relative to controls (0-µg/mL fractions and 0 ng LPS/mL). Within a row, means without a common superscript letter are significantly different from each other, P < 0.05. CON, control; DP-Fr, dried plum fraction.