FIG. 6.
Nano-pulsed laser therapy (NPLT) decreases microglia activation 10 days after blast injury. (A) Representative images of brain sections at the level of the somatosensory cortex (bregma level: −4.16 mm) stained with an antibody against CD68 (a marker of activated microglia; red). Nuclei are counterstained with DAPI and are shown in blue. Calibration bar is 50 μm. (B) Quantification of the number of CD68pos cells in the cortex. N = 3 rats/group. *p < 0.05; **p < 0.01 one-way analysis of variance (ANOVA) followed by post hoc Tukey's multiple comparisons test. (C) Representative coronal view of the rat brain at −3.80 mm of bregma showing the location of CD68pos cells (shaded area) identified by immunofluorescence in the brain of BLAST and BLAST+NPLT rats (modified from Paxinos, G., and Watson, C. The Rat Brain in Stereotaxic Coordinates, 4th ed. Academic Press: San Diego, Fig. 35).