Table 1.
The First Model Involving Removal of Intestinal Tissue, Implantation into the Omentum, and Finally Anastomosis in Continuity with the Small Bowel
| Animal | Gender | Surgeries | Age at surgeries (days) | Age at euthanasia (days) | Euthanasia reason | Weights (kg) | Complications | Construct outcome |
|---|---|---|---|---|---|---|---|---|
| 1 (228) | Female | (1) 10 cm SBR | 4 | 1.4 | (1) Wound infection | |||
| (2) 5-Day organoid seeded PEVA scaffold in omentum | 9 | 1.9 | (2) Wound dehiscence and hernia postoperatively | |||||
| (3) Exploration, no scaffold found, euthanasia | 54 | 54 | No construct for anastomosis | 9.4 | Not present | |||
| 2 (226) | Female | (1) 10 cm SBR | 4 | 1.46 | ||||
| (2) 5-Day organoid seeded PEVA scaffold in omentum | 9 | 1.9 | (2) Small bowel injury, SBR, and anastomosis during operation; wound dehiscence and hernia postoperatively | |||||
| (3) Placed construct in continuity, rectopexy, hernia repair | 44 | 6.1 | Rectal prolapse occurred, age 39–44 days | |||||
| 49 | Recurrent rectal prolapse | Stricture at construct anastomosis | ||||||
| 3 (225) | Female | (1) 10 cm SBR | 4 | 1.86 | ||||
| (2) 5-Day organoid seeded PEVA scaffold in omentum | 9 | 2.4 | (2) Wound dehiscence and hernia postoperatively | |||||
| (3) Exploration, attempted anastomosis of construct to small bowel, but construct separated from fibrous capsule; euthanasia | 37 | 37 | Construct without ability to anastomose; prolonged anesthesia time | 7.1 | Construct separated from fibrous omental capsule | |||
| 4 (227) | Female | (1) 10 cm SBR | 4 | 1.24 | ||||
| (2) 5-Day organoid seeded PEVA scaffold in omentum | 9 | 1.9 | (2) Scaffold had bacterial colonization on implantation; wound infection, wound dehiscence, hernia | |||||
| Euthanasia | 29 | Found down; hypoglycemic | 3.0 | Construct explanted from omentum | ||||
| 5 (229) | Female | (1) Unseeded scaffold implantation into omentum | 4 | 1.6 | ||||
| (2) Exploration of scaffold | 22 | 4.5 | Scaffold not well incorporated/encapsulated at this time | |||||
| (3) Reexploration of scaffold, euthanasia | 54 | 54 | Decision to change scaffold material due to poor incorporation of PEVA scaffolds | 12.4 | Vascular imaging with cardiogreen, explantation of scaffold |
The scaffolds used in these animals were made of PEVA.
SBR, small bowel resection; PEVA, poly(ethylene-co-vinyl acetate).