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. 2018 May 8;19(Suppl 4):99. doi: 10.1186/s12859-018-2068-7

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — A flow chart showing the design of this study. Three gene expression profiles and two methylation profiles were retrieved from two studies on two social insect species, Polistes canadensis and Dinoponera quadriceps. All of them were processed by PCA-based unsupervised FE, whereas two pairs of gene expression and methylation profiles were analyzed by TD-based unsupervised FE. Differential expression between castes was analyzed in seven obtained gene sets. Analysis of GO term enrichment was performed on three sets of genes derived from gene expression analysis and on two sets of genes generated by TD-based unsupervised FE. The full list of the selected genes is presented in Additional file 1