Figure 4.

Localization of CREB binding sites in the let-7f pri-miRNA promoter. A) Potential CREB binding sites in the 5′-upstream region of pri-let-7f as identified by JASPER. B) Chromatin immunoprecipitation results using a CREB antibody and pri-let-7f primers for PCR (see Materials and Methods). Sites 1, 2, and 3 were identified as CREB binding sites based on the presence of a PCR product. Results are representative of 4 experiments performed. IgG was utilized as a negative control in the immunoprecipitation step. The input refers to cell extracts prior to immunoprecipitation and the primers were validated by the presence of PCR products.