Figure 6.

Bone formation and resorption analyses in PTH- and vehicle-treated KO and WT mice. A–C) Serum was collected at the time of euthanasia, and P1NP, TRAcP 5b, and CTX-I were measured via ELISA (n = 10–11/group). D, E) Calcein (30 mg/g, i.p.) was administered 5 and 2 d before euthanasia. Undecalcificed tibiae were collected, fixed, and embedded, and sections were analyzed for BFR/BS and MAR. PTH treatment increased BFR/BS and MAR in both KO and WT. F) TRAP-stained, paraffin-embedded tibiae sections were quantified for TRAP⁺ multinucleated cells. KO mice treated with PTH showed decreased N.Oc/BS vs. vehicle-treated KO mice. Data are means ± sem (n = 8–11/group). *P < 0.05, **P < 0.01, ***P < 0.001.