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. 2018 Apr 18;28(7):2577–2593. doi: 10.1093/cercor/bhy082

Figure 3.

Figure 3.

Altered cortical interneuron numbers at mid and late stages of cortical development in the Vegfa120/120 mutant forebrain. (A) In situ hybridization for the interneuron-marker Gad67 in the cortex of E14.5 Vegfa+/+ and Vegfa120/120 mouse forebrains. (B) Quantification of interneuron number at E14.5. Data represented as mean values ± SEM (**P ≤ 0.01, ***P ≤ 0.001; t-test; n = 3 for each). (C) In situ hybridization for the interneuron-specific Lhx6 mRNA in the cortex of E18.5 Vegfa+/+ and Vegfa120/120 mouse forebrains with dotted lines delineating the LGE VZ/SVZ and the ventral striatum in high magnification images of the subpallium, corresponding to regions where an ectopic increase in Lhx6+ interneurons is observed, and with a reduction in interneurons observed in high magnification of the dorsal cortex. (D) Bar charts show mean total number of Lhx6+ interneurons quantified in cortical regions and within the subpallium at E18.5 (t-test, n = 3 for each; **P ≤ 0.01, ***P ≤ 0.001).