Figure 2.

Immunofluorescence double staining of PHB1 Tg brain. (a) Confocal images of coronal sections of a PHB1 Tg mouse (10 weeks old) doubly stained with GFP (green, as PHB1 transgene expression marker) and NeuN (red, as neuronal marker). Merged images show that GFP positive cells are also NeuN positive, suggesting neuronal specific expression of the transgene. (b) Confocal images of double staining of GFP and GFAP (red, astrocyte marker). Merged panels show no co-localization between GFP and GFAP positive cells. Top panels in both (a) and (b) are images acquired with a low magnification lens (5×); lower panels were acquired with a higher magnification lens (63×) from the regions indicated by the square in the top panels. Size bars are 500 um and 25 um as indicated.