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. Author manuscript; available in PMC: 2018 Jun 13.
Published in final edited form as: Electrophoresis. 2013 May 8;34(11):1701–1709. doi: 10.1002/elps.201200632

Table 1. Validation data for selected amino acids in human plasma with the optimized CE-LIF method.

L-proline L-isoleucine L-ornithine L-glutamine L-alanine L-threonine glycine L-serine D-serine L-glutamate
Linearity (N=20)
Slope 0.0078 ± 0.0003 0.0059 ± 0.0004 0.0017 ± 0.0002 0.0048± 0.0003 0.00382 ± 8.4E-05 0.0052 ± 0.0003 0.0059 ±0.0004 0.0055 ± 0.0003 0.0047 ± 0.0002 0.00481 ± 0.00003
Intercept −0.075 ± 0.099 −0.053 ± 0.067 −0.020 ± 0.028 −0.37 ± 0.27 −0.028 ± 0.038 −0.19 ± 0.12 −0.012 ± 0.0179 −0.21 ± 0.13 0.0004 ± 0.003 0.012 ± 0.012
r 0.997 0.992 0.991 0.997 0.999 0.997 0.995 0.996 0.998 0.9997
Range (μM) 25-300 25-300 25-300 125-1500 25-750 25-600 25-750 25-750 0.5-25 25-300
Accuracy
% 97.1 101.5 97.1 101.8 94.0 96.0 96.7 103.7 100.9 92.0
RSD(%) 3.7 5.2 6.9 4.5 7.76 7.3 6.0. 6.2 4.5 9.8
Instrumental precisión (N=12) RSD(%) Method Precision Standard 4.3 2.2 4.3 5.2 1.4 5.9 4.3 5.6 4.8 1.6
Inter-assay (N=12) RSD (%) 6.5 4.2 6.8 6.2 3.8 5.5 5.2 6.7 4.4 2.8
Intra-assay (N=6) RSD (%) Method Precision Sample 3.1 3.5 2.9 3.2 3.6 3.7 3.7 3.3 2.7 3.0
Inter-assay (N=12) RSD (%) 6.0 5.3 6.0 5.7 5.8 5.5 4.4 5.3 6.8 3.4
Intra-assay (N=6) RSD (%) 5.4 5.2 4.0 5.6 2.0 2.9 2.8 5.1 5.0 2.0
LOQ (EURACHEM)(nM) 738 630 1.44 μM 146 889 714 128 174 231 203
LOD(nM) 221 189 433 43.7 267 214 38.3 52.2 69.5 61.0