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. Author manuscript; available in PMC: 2018 Jun 13.
Published in final edited form as: Biomaterials. 2016 Apr 1;97:85–96. doi: 10.1016/j.biomaterials.2016.03.039

Figure 1.

Figure 1

MPLA on the surface of NP, mimicking bacterial cell walls (A), increases BMDC uptake, pro-inflammatory cytokine production, and antigen-specific T cell proliferation. (B) BMDCs incubated with titrated PLGA NP internalize MPLA-coated particles more avidly than blank NP (*p<.0001 for all concentrations) (C) After 24h with NP, BMDCs produce a higher amount of pro-inflammatory cytokines IL-6 (***p=.0001), (D) IL-12 (*p=.0217), and (E) IL-10 (****p<.0001) in response to MPLA/OVA vs. −/OVA NP. (F) Co-incubating OVA-specific OT-I splenocytes with NP-treated BMDCs results in greater production of proliferative cytokines IFN-gamma (*p<.05, **p<.01) (G) and IL-2 (****p<.0001) after 5 days. Results are representative of three independent experiments. Error bars represent Standard Experimental Error (SEM).