Supplementary Table 1.
Relative expression levels of proteins on western blotting assays.
| Protein | Group | ||||
|---|---|---|---|---|---|
| Control | NC | LPS+NC | LPS+ROCK1 | LPS+Y-27632 | |
| ROCK1 | 1.00±0.00 | 1.07±0.15 | 3.33±0.48a | 5.98±0.86ab | 1.07±0.15bc |
| RhoA | 1.00±0.00 | 0.98±0.14 | 2.36±0.34a | 4.47±0.64ab | 1.21±0.17bc |
| TLR4 | 1.00±0.00 | 0.90±0.13 | 3.91±0.41a | 5.46±0.65ab | 1.59±0.23abc |
| p-IκBα | 1.00±0.00 | 1.05±0.15 | 4.39±0.63a | 7.96±1.14ab | 2.40±0.35abc |
| IκBα | 1.00±0.00 | 0.98±0.14 | 0.35±0.02a | 0.17±0.01ab | 0.68±0.08abc |
| NF-κB p65 (plasma) | 1.00±0.00 | 0.99±0.14 | 0.41±0.06a | 0.19±0.03ab | 0.65±0.05abc |
| NF-κB p65 (nuclear) | 1.00±0.00 | 0.94±0.14 | 3.78±0.40a | 5.13±0.62ab | 2.50±0.40abc |
| p-ERK1/2 | 1.00±0.00 | 0.90±0.13 | 4.83±0.69a | 9.21±1.32ab | 2.00±0.29abc |
| ERK1/2 | 1.00±0.00 | 0.94±0.14 | 1.00±0.15 | 0.97±0.14 | 0.95±0.14 |
| p-JNK | 1.00±0.00 | 0.89±0.13 | 2.44±0.27a | 5.48±0.79ab | 1.29±0.19abc |
| JNK | 1.00±0.00 | 1.04±0.15 | 0.94±0.13 | 0.87±0.13 | 1.11±0.16 |
| p-p38 | 1.00±0.00 | 0.97±0.14 | 2.14±0.31a | 3.34±0.48ab | 1.30±0.19bc |
| p38 | 1.00±0.00 | 0.95±0.14 | 1.02±0.15 | 0.95±0.14 | 1.08±0.15 |
| Bax | 1.00±0.00 | 1.06±0.15 | 1.98±0.27a | 3.77±0.54ab | 1.13±0.11bc |
| Bcl-2 | 1.00±0.00 | 0.96±0.14 | 0.50±0.06a | 0.25±0.02ab | 0.77±0.11abc |
a
p<0.05 versus NC group;
b
p<0.05 versus LPS+NC group;
c
p<0.05 versus LPS+ROCK1 group.
Data were expressed as mean ± standard deviation. ROCK1, Rho kinase type 1. TLR4, toll-like receptor 4. IκBα, I κ B proteins α. NF-κB p65, nuclear factor of κ B subunit p65. ERK1/2, extracellular signal-regulated kinase 1/2. JNK, c-Jun N-terminal kinase.