Figure 6.

Genome editing of cultivars by crossing Cas9‐sgRNA lines. (a) Selection of the natural outcrossing plants from open‐pollination progeny of HY. Plants with serrated leaves are hybrids of HY and the double homozygous mutant SCLV3‐35 for the incompletely dominant lobed‐leaf trait. (b) The read numbers at the S1 site of BnCLV3 in mixed genomic DNA from F₁ hybrid plants with T‐DNA are shown, and WT (HY) was included as a control. (c) Frequency of different genotype reads. Original mutant reads, reads with the same mutations detected in SCLV3‐35; novel mutant, reads with different mutations detected in SCLV3‐35.