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Detection of potential off‐target effects for each sgRNA target site in T0 mutated plants
| On‐target site | Binary vector | No. of plants for sequencing | Putative off‐target sites | Off‐target editing |
|---|---|---|---|---|
| S1 | SCLV3 + UCLV3 | 47 | 17 | No |
| S3 | SCLV1 | 22 | 18 | No |
| S4 | SCLV1 | 22 | 11 | No |
| S7 | SCLV2 | 45 | 4 | No |
| S8 | SCLV2 | 45 | 7 | No |
