Fig 3. Fungal species analysis using either the T-RFLP or conventional culture methods on duodenal fluid samples from patients with liver cirrhosis and controls (n = 56).

Each bar represents a single species. A star indicates that a fungal species could not be confirmed by the alternative method. The x-axis presents single duodenal samples so that multiple culture or T-RFLP-identified pathogens per sample are stacked in columns. Samples with culture-positive and PCR/T-RFLP-positive samples are compared in A (n = 40). Culture-negative but PCR/T-RFLP-positive samples are displayed in B (n = 9) and culture-positive and PCR/T-RFLP-negative samples are presented in C (n = 2). The duodenal samples that were culture-positive and PCR-positive but T-RFLP-negative are represented in area D (n = 5). Additional duodenal samples that were culture negative but PCR-positive with DNA not being sufficient for T-RFLP analysis (n = 3) have been left out in analysis.