Table 2. Fungal pathogen and Candida DNA detection rates from microbial culture, real-time PCR analysis, and its statistical significance.
Patients with cirrhosis (n = 135) | Control patients without cirrhosis (n = 26) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Sample material | No. of samples | Culture positive (%) | PCR positive (%), median copies/ml (range) | P value culture vs. PCR | No. of samples | Culture positive (%) | PCR positive (%), median copies/ml (range) | P value culture vs. PCR | P value PCRcirrhosis vs. PCRcontrol |
Blood | n = 135 | n = 1 (0.7%) | n = 1a (0.7%), 3.0x101 | n = 26 | n = 0 (0%) | n = 0 (0%) | |||
Ascites | n = 92 | n = 0 (0%) | n = 1 (1.1%), 1.3x101 | ||||||
Duodenal fluid | n = 54 | n = 36 (66.7%) | n = 44b (81.5%), 3.8x105 | 0.123 | n = 26 | n = 12 (46.2%) | n = 16c (61.5%) 1.2x105 | 0.404 | 0.096 |
(2.3x102-6.3x109) | (2.5x102-2.4x107) |
a1/1
b34/36
c12/12 positive PCR results confirming microbial culture