Fig 5. Proliferation and expression of myogenic regulatory factors MyoD and myogenin.

(A) Proliferation was assessed using 5-ethynil-2'-deoxyuridine (EdU⁺) following 6, 24, 48, and 72 hours in a growth medium. Myogenic regulatory factors (B) MyoD⁺ and (C) Myogenin⁺ nuclei were assessed following 24 and 72 hours in a differentiation medium. Representative images of (D) MyoD⁺ and (E) Myogenin⁺ nuclei at 24 hours. Scale bar = 50μm. * Significance p < 0.05.